Tianxianteng
1、 Pharmacopoeia standard of tianxianteng       This product is the dry aboveground part of Aristolochia debilis sieb.et zucc. or Aristolochia contorta bge., a plant of Aristolochiae family. It is harvested in autumn, removed impurities, and dried in the sun.
      [character] the stem of this product is slender cylindrical, slightly twisted, with a diameter of 1-3mm; the surface is yellowish green or yellowish brown, with longitudinal edges and nodes, and the internodes are unequal in length; It is brittle and easy to break. There are several vascular bundles of different sizes on the cross section. The leaves are alternate, mostly shrunken and broken. After flattening, the complete leaves are triangular, narrow ovate or triangular, wide ovate, heart-shaped at the base, dark green or yellowish brown, with obvious basal veins and slender petioles. It smells fresh and tastes light.
      [identification] (1) the transverse section of the stem of this product: the epidermal cells are square and covered with cuticle. The cortex is narrower. . There are several vascular bundles with different sizes. The cambium forms a ring. The catheter is round, with a diameter of 10-170 μ M. Myelinated.
      (2) Take 3G of this product powder, add 50ml of ethanol, heat and reflux for 1 hour, filter, evaporate the filtrate, add 5ml of ethanol to dissolve the residue as the test solution. Another 3G of tianxianteng reference medicinal material was prepared into the reference medicinal material solution by the same method. According to the test of thin-layer chromatography (general rule 0502), suck 5 μ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use the upper solution of toluene ethyl acetate water formic acid (35:30:1:1) as the developing agent, develop, take out, dry, and check under the UV light (365nm). In the chromatogram of the test sample, spots of the same color appear on the corresponding position of the chromatogram of the control medicinal material.
      [check] moisture   Not more than 10.0% (the second method of general rule 0832).
      Total ash   Not more than 9.0% (general rule 2302).
      Aristolochic acid I limit   Determine according to high-performance liquid chromatography (general rule 0512).
      ; Octadecylsilane bonded silica gel was used as filler; Acetonitrile was used as mobile phase a, and 1% glacial acetic acid solution -0.3% triethylamine solution (10:1) was used as mobile phase B. gradient elution was performed according to the following table; The detection wavelength was 250nm. The number of theoretical plates should not be less than 7000 according to aristolochic acid I peak.
         --------------------------------------------
              Time (minutes)         ;       Mobile phase B (%)
         --------------------------------------------
         0～13            35               65
         13～14           35→45           65→55
         14～27           45→47           55→53
         27～28           47→100          53→0
         --------------------------------------------
      Preparation of reference solution   Take an appropriate amount of aristolochic acid I reference substance, accurately weigh it, add methanol to make a solution containing 1.0 μ g per 1ml.
      Preparation of test solution   Take about 2G of this product powder (passing through No. 3 screen), weigh it precisely, place it in a corked conical flask, add 50ml of methanol precisely, close the stopper, weigh it, sonicate (power 250W, frequency 33khz) for 30 minutes, cool it, weigh it again, make up the lost weight with methanol, shake it well, filter it, and take the filtrate.
      ; Precisely suck 20 μ l of the reference solution and 20 μ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
      .
      [extract] determined by the hot leaching method under the determination method of water-soluble extract (general rule 2201), it shall not be less than 16.0%.
      Decoction pieces
      [processing] remove impurities and cut into sections.
      [nature, taste and meridian tropism] bitter, warm. It belongs to the liver, spleen and kidney meridians.
      [functions and indications] promote qi and blood circulation, unblock collaterals and relieve pain. .
      [usage and setting] 3 ～ 6G.
      [note] this product contains aristolochic acid, which can cause adverse reactions such as kidney damage; Use with caution for children and the elderly; .
      [storage] put it in a dry place.


2、 Chemical constituents of tianxianteng
TianxiantenghanAristolochic acid D（aristolochic acid），Magnoliane(magnoflorine) andβ - sitosterol（β-sitosterol）。

3、 Pharmacological effects of tianxianteng
Promoting qi and blood circulation; Diuresis and detumescence; Detoxification. Main stomach pain; Colic pain; Edema of pregnancy; ; Snakebite