Xiangjiapi
1、 Pharmacopoeia standard of cortex Periplocae       This product is the dried root bark of Periploca sepium bge. Excavate in spring and autumn, peel root bark and dry in the sun.
      . The inner surface is light yellow or light yellowish brown, relatively smooth, with fine longitudinal lines. It is light, brittle, easy to break, with irregular section and yellowish white. It has special aroma and bitter taste.
      [identification] (1) the powder of this product is light brown. The diameter of calcium oxalate square crystal is 9 ～ 20 μ M. Stone cells are rectangular or polygonal, with a diameter of 24-70 μ M. . Cork cells are brownish yellow and polygonal. There are many starch grains, single round or oblong, with a diameter of 3-11 μ M; The compound granule is composed of 2-6 fractions.
      (2) Take 10g of this product powder, put it into a 250ml flask, add 150ml of water, heat and distill. The distillate has a specific aroma. Collect 10ml of the distillate, and put it into two tubes. Add 1 drop of 1% ferric chloride solution to one tube, which shows a reddish brown color; Add 5ml of saturated solution of hydrazine sulfate and a small amount of sodium acetate to crystallize in the other tube, heat it slightly and cool it to generate yellowish green precipitate, and observe it under ultraviolet light (365nm), showing strong yellow fluorescence.
      (3) Take 1g of this product powder, add 10ml of ethanol, heat and reflux for 1 hour, filter, put the filtrate into a 25ml volumetric flask, add ethanol to the scale, shake well, accurately measure 1ml, put into a 20ml volumetric flask, add ethanol to the scale, shake well, measure according to UV visible spectrophotometry (general rule 0401), and there is a maximum absorption at the wavelength of 278nm.
      (4) Take 2G of this product powder, add 30ml of methanol, heat and reflux for 1 hour, filter, evaporate the filtrate, add 2ml of methanol to dissolve the residue, and use it as the test solution. . According to the test of thin-layer chromatography (general rule 0502), suck 2 μ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use petroleum ether (60 ～ 90 ℃) - ethyl acetate glacial acetic acid (20:3:0.5) as the developing agent, develop, take out, dry, and spray dinitrophenylhydrazine test solution. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
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      Acid insoluble ash content shall not exceed 4.0% (general rule 2302).
      [extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), using dilute ethanol as solvent, it shall not be less than 20.0%.
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol water acetic acid (70:30:2); .
      Preparation of internal standard solution take appropriate amount of butyl p-hydroxybenzoate, weigh accurately, add 60% methanol to make a solution containing 6mg per 1ml.
      Determination method take an appropriate amount of 4-methoxysalicylaldehyde reference substance, accurately weigh it, place it in a brown measuring flask, add 60% methanol to make a solution containing 1mg per 1ml. Precisely measure 4ml of the solution and 2ml of the internal standard solution, put them into a 25ml volumetric flask, add 60% methanol to the scale, shake well, suck 20 μ L, inject them into the liquid chromatograph, and record the chromatogram; In addition, take about 0.25 ～ 0.5g of the crude powder of this product, dry it at 60 ℃ for 4 hours, weigh it accurately, put it into a 50ml flask, add 15ml of 60% methanol, heat and reflux for 1.5 hours, filter it, put the filtrate into a 25ml volumetric flask, wash the container with a small amount of 60% methanol, filter the washing solution into the same volumetric flask, add 2ml of internal standard solution precisely, add 60% methanol to the scale, shake it well, filter it, and take the continued filtrate as the test solution. .
      The product shall be dried at 60 ℃ for 4 hours, and the content of 4-methoxysalicylaldehyde (C8H8O3) shall not be less than 0.20%.
      Decoction pieces
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      This product is irregular thick film. The outer surface is grayish brown or yellowish brown, and the cork is often scaly. The inner surface is yellowish or yellowish brown with fine longitudinal lines. The cut surface is yellowish white. It has special aroma and bitter taste.
      [identification], [inspection], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] pungent, bitter and warm; . Return to liver, kidney and heart meridian.
      . It is used for edema of lower limbs, palpitations, shortness of breath, wind cold and dampness, and soreness of waist and knees.
      [usage and dosage] 3 ～ 6G.
      [note] do not overdose.
      [storage] store in a cool and dry place.


2、 Chemical constituents of cortex Periplocae
The root bark contains steroidal glucosides:PeriplocatinPeriplocin, namely Periplocoside g, Periplocoside k[1], h1[2], h2[3], a, B, c[4], D, e, lEleutheroside of Acanthopanax senticosusM、Eleutheroside of Acanthopanax senticosusN[5]、J、K、F、O[6]， Periploside a, B, c[7],Periplocatin(periplocymarin) [8], β - sitodteryl - β - D-glucoside [11]; , 21-tetraol-20-one (21-o-methyl-5-pregnene-3 β, 14 β, 17 β, 21-tetrol-20-one) a[9], and also contains Periplocae oligosaccharide C1, C2, F1, f2[10], 4-methoxy-salicylaldehyde andβ - sitosterol(β-sitlsterol)[11]

3、 Pharmacological effects of cortex Periplocae
1. cardiotonic effect: Periploca Periploca preparation (a water-soluble substance extracted with ether and ethanol) stops the eutopic frog heart in systole, increases the blood pressure of eutopic cat heart, enhances cardiac contractility, and increases the minute output of failing cat heart (cardiopulmonary device). The skin extract B (chloroform and ethanol extract) has also been proved to have general cardiac glycoside effect in the eutopic cat heart and ECG experiments. Asclepiadoside and peripolocymarin from the root bark of periplocagraeca L. have rapid action, short duration and weak accumulation effect.
2. sedative effect: Periploca Periploca bark extract B has no sedative effect on mice. Periploca tincture and Periploca solution show central excitation, which may be caused by its volatile components.
3. other effects: asclepiadoside can increase pulmonary circulation under certain conditions. In clinic, cardiotonic Tongfang has diuretic effect. In addition, Periploca pericarp has insecticidal effect.
4. biological activity: the cat unit of Periploca Periploca bark preparation is 0.176g of crude drug. Periploca extract B is 1.6mg/kg. Asclepiadoside LG contains 5780 cat units, with low accumulation and shorter action time than that of strobiloside G.