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Quantitative Analysis of Anthraquinones in Cassiae Semen by Processing Method

Journal name:KPUBS
Literature No.:
Literature Url: http://www.kpubs.org/article/articleMain.kpubs?articleANo=HKSOBF_2014_v45n3_200
Date publication:July 30, 2014

Quantitative Analysis of Anthraquinones in Cassiae Semen by Processing Method

Abstract

In this study, we performed quantification determination of four major components including aurantio-obtusin, emodin, chrysophanol, and physcion in the 70% ethanol extracts of non-processed Cassiae Semen and processed Cassiae Semen using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the 4 constituents used a Gemini C 18 column kept at 40℃ by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 μL. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in non-processed Cassiae Semen were 0.07%, 0.02%, 0.25%, and 0.10%, respectively. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in processed Cassiae Semen were 0.04-0.14%, 0.01-0.03%, 0.02-0.42%, and 0.01-0.24%, respectively. Consequently, the optimal processing condition of Cassiae Semen for the improvements of amounts of four anthraquinone compounds was obtained by roasting at 240℃ for 15 min.

 aurantio-obtusin, emodin chrysophanol Biopurify Biochemical
Ltd.(Chengdu, China